hMET/LL/2
CBPG0146
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| I. Background | |||
| C-Met 屬于受體酪氨酸激酶家族，是一種異二聚體，由高度糖基化的細(xì)胞外 50 kDa α 鏈和跨膜 140 kDa β鏈組成，通過二硫鍵連接在一起。作為一種天然的 HGF 受體，它表達(dá) 于各種上皮細(xì)胞的表面，并促進(jìn)腫瘤的發(fā)生。與 HGF 結(jié)合后，兩個(gè)亞基二聚化，導(dǎo)致兩個(gè) 酪氨酸殘基（Tyr1234 和 Tyr1235 ）自磷酸化。酪氨酸殘基的自磷酸化導(dǎo)致一個(gè)激活的多功 能停靠位點(diǎn)，該位點(diǎn)由 SH2 結(jié)構(gòu)域、PTB 結(jié)構(gòu)域和 Met 結(jié)合結(jié)構(gòu)域 （MDB） 組成，該結(jié) 構(gòu)域募集接頭分子。 | |||
| II. Introduction | |||
| Host Cell: | LL/2 | ||
| Expressed gene: | hMET | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | DMEM+10%FBS+10 μg/ml blasticidin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus, mouse | ||
| Tissue: | lung | ||
| Morphology: | rounded - loosely attached or floating | ||
| Growth Properties: | mixed, adherent and suspension | ||
| IV. Representative Data | |||
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Figure 1. Recombinant hMET/LL/2 stably expressing hMET. |
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