IRB Effector Reporter Cell
CBP74296
| I. Background | |
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胰島素受體（INSR，Insulin Receptor）是調(diào)控葡萄糖代謝、細(xì)胞生長(zhǎng)及能量穩(wěn)態(tài)的核 心跨膜受體。INSR 由兩個(gè)胞外α亞基（配體結(jié)合域）和兩個(gè)跨膜β亞基（激酶活性域）通過(guò) 二硫鍵連接成四聚體（α2β2）。胰島素結(jié)合α亞基后，觸發(fā)β亞基自磷酸化并激活其酪氨酸 激酶活性，進(jìn)而通過(guò) PI3K-AKT 和 MAPK/ERK 信號(hào)通路協(xié)調(diào)代謝與增殖功能。INSR作為代謝與增殖信號(hào)的“分子開(kāi)關(guān)”，在糖尿病、癌癥及罕見(jiàn)遺傳病中具有核心地位。 |
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| II. Description | |
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IRB Effector Reporter Cell 報(bào)告基因藥靶模型很好的模擬了體內(nèi) Insulin 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程， 原理見(jiàn)下圖所示。
Figure 1. INSR Effector Reporter Cell 細(xì)胞模型原理圖
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| III. Introduction | |
| Expressed gene: | IRB |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+200 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 1. Recombinant IRB Effector Reporter Cell stably expressing IRB.  Figure 2. Dose Response of Recombinant Human Insulin in IRB Effector Reporter Cell (C23). |
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